Batimastat (BB-94)
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Batimastat (BB-94)是一种合成的有效MMPs抑制剂,作用于MMP-1(interstitial collagenase)、MMP-2(72 kDa type IV collagenase)、MMP-9(92 kDa type IV collagenase)、MMP-7(matrilysin)和MMP-3(Stromelysin 1),作用的IC50值分别为3 nM、4 nM、4 nM、6 nM和20 nM。Batimastat是一个低分子量(MW = 478)的多肽样胶原蛋白底物类似物,由一个多肽骨架和一个异羟肟酸基团组成,分别与MMPs和具催化活性的Zn原子结合。在包括卵巢癌异种移植肿瘤和人结肠癌在内的多个肿瘤模型中,Batimastat展示了抗肿瘤和抗血管生成的活性。
参考文献:
1. Bernard Davies, Peter D. Brown, Nick East, Michael J. Crimmin, and Frances R. Balkwill. A synthetic matrix metalloproteinase inhibitor decreases tumor burden and prolongs survival of mice bearing human ovarian carcinoma xenografts. Cancer Research 1993; 53: 2087-2091
2. X. Wang, X. Fu, P.D. Brown, M. J. Crimmin, and R. M. Hoffman. Matrix metalloproteinase inhibitor BB-94 (batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice. Cancer Research 1994; 54: 4726-4728
3. Raffaella Giavazzi, Angela Garofalo, Cristina Ferri, Valeria Lucchini, Elisabeth A. Bone, Stefania Chiari, Peter D. Brown, M. Ines Nicoletti, and Giulia Taraboletti. Batimastat, a synthetic inhibitor of matrix metalloproteinases, potentiates the antitumor activity of cisplatin in ovarian carcinoma xgenografts. Clinical Cancer Research 1998; 4: 985-992
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Physical Appearance | A solid |
Storage | Store at 4°C |
M.Wt | 477.64 |
Cas No. | 130370-60-4 |
Formula | C23H31N3O4S2 |
Synonyms | Batimastat, BB-94 |
Solubility | ≥23.88 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
Chemical Name | (2S,3R)-N1-hydroxy-3-isobutyl-N4-((S)-1-(methylamino)-1-oxo-3-phenylpropan-2-yl)-2-((thiophen-2-ylthio)methyl)succinamide |
SDF | Download SDF |
Canonical SMILES | CNC([C@@H](NC([C@@H]([C@H](CSC1=CC=CS1)C(NO)=O)CC(C)C)=O)CC2=CC=CC=C2)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验: | |
细胞系 |
C170HM2和AP5LV细胞系 |
溶解方法 |
在DMSO中的溶解度 >10 mM。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
96 h;3.0 μg/ml |
应用 |
在batimastat存在时的细胞增殖实验用MTT吸光度法进行评估。在无血清和含scrum培养基中培养的C170HM2和AP5LV细胞中,batimastat对细胞生长没有显著影响。 |
动物实验: | |
动物模型 |
人结肠癌原位移植裸鼠模型 |
剂量 |
30 mg/kg;i.p. |
应用 |
BB-94引起原发肿瘤的中位重量从对照组中的293 mg(范围: 1141~124 mg)减少到BB-94治疗组中的144 mg(范围: 424~38 mg)(P <0.001),并显著减少肿瘤侵入邻近组织的发生率,从对照组中发生率为67%(12/18)减少到BB-94治疗组中的35%(7/20)。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。 |
References: [1] Watson S A, Morris T M, Robinson G, et al. Inhibition of organ invasion by the matrix metalloproteinase inhibitor batimastat (BB-94) in two human colon carcinoma metastasis models[J]. Cancer research, 1995, 55(16): 3629-3633. [2] Wang X, Fu X, Brown P D, et al. Matrix metalloproteinase inhibitor BB-94 (batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice[J]. Cancer research, 1994, 54(17): 4726-4728. |
描述 | Batimastat (BB-94)是一种有效的广谱基质金属蛋白酶(matrix metalloprotease,MMP)抑制剂,作用于MMP-1、MMP-2、MMP-9、MMP-7和MMP-3,IC50值分别为3 nM、4 nM、4 nM、6 nM和20 nM。 | |||||
靶点 | MMP-1 | MMP-2 | MMP-9 | MMP-7 | MMP-3 | |
IC50 | 3 nM | 4 nM | 4 nM | 6 nM | 20 nM |
质量控制和MSDS
- 批次:
化学结构
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