AZD2858
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AZD2858是一种GSK-3选择性抑制剂,IC50值为68 nM.
糖原合酶激酶3β(GSK-3β)在典型的Wnt通路中扮演重要角色,且参与多个生物学过程[1].
在人类成骨细胞中,AZD2858可短暂提高β连环蛋白水平[1].hADSC与AZD2858孵育可显著提高成骨矿化作用.这表明抑制GSK-3可提高hADSC的成骨分化[2].
大鼠口服AZD2858疗法两周后可以剂量依赖的方式增加骨小梁量.在皮质位点也有显著效果.在生物力学测试中,脊椎抗压强度和股骨骨干力量均有提高.此外,骨形成指数有显著提高,骨形成及骨吸收的血清标记均有增加[1].另外,AZD2858可驱动间充质细胞转变为成骨细胞,使得在不稳定骨折环境中发生直接骨修复[3].
参考文献:
[1]. Marsell R, Sisask G, Nilsson Y, et al. GSK-3 inhibition by an orally active small molecule increases bone mass in rats. Bone, 2012, 50(3): 619-627.
[2]. Gilmour PS, O'Shea PJ, Fagura M, et al. Human stem cell osteoblastogenesis mediated by novel glycogen synthase kinase 3 inhibitors induces bone formation and a unique bone turnover biomarker profile in rats. Toxicol Appl Pharmacol, 2013, 272(2): 399-407.
[3]. Sisask G, Marsell R, Sundgren-Andersson A, et al. Rats treated with AZD2858, a GSK3 inhibitor, heal fractures rapidly without endochondral bone formation. Bone, 2013, 54(1): 126-132.
Storage | Store at -20°C |
M.Wt | 453.52 |
Cas No. | 486424-20-8 |
Formula | C21H23N7O3S |
Solubility | insoluble in H2O; insoluble in EtOH; ≥11.35 mg/mL in DMSO |
Chemical Name | 3-amino-6-(4-((4-methylpiperazin-1-yl)sulfonyl)phenyl)-N-(pyridin-3-yl)pyrazine-2-carboxamide |
SDF | Download SDF |
Canonical SMILES | NC1=C(C(NC2=CC=CN=C2)=O)N=C(C3=CC=C(S(=O)(N4CCN(C)CC4)=O)C=C3)C=N1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
![AZD2858](http://www.apexbt.com//media/diy/images/struct/B1537.png)