AM 114
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AM 114是硼酸查尔的衍生物,一种有效的小分子蛋白酶体抑制剂,抑制20S蛋白酶体的糜蛋白酶样活性,IC50值接近1 μM,可在不明显破坏p53和MDM2蛋白相互作用的情况下导致全细胞中泛素化p53和其他细胞内蛋白的大量积累。AM 114也具有抗癌活性,可有效抑制人结肠癌HCT116细胞的生长,在MTT和集落形成试验中的IC50值分别为1.5 μM和0.6μM [1]。
参考文献:
[1] Achanta G, Modzelewska A, Feng L, Khan SR, Huang P. A boronic-chalcone derivative exhibits potent anticancer activity through inhibition of the proteasome. Mol Pharmacol. 2006 Jul;70(1):426-33. Epub 2006 Apr 24.
- 1.Gunderwala A, Porter J. "A luminescence assay for natural product inhibitors of the Mycobacterium tuberculosis proteasome." Phytochem Anal. 2016 Mar;27(2):126-32. PMID:26778282
- 2.Chen, Wei, et al. "Competitive Expression of Endogenous Wheat CENH3 May Lead to Suppression of Alien ZmCENH3 in Transgenic Wheat× Maize Hybrids." Journal of Genetics and Genomics (2015).
Physical Appearance | A solid |
Storage | Store at 4°C |
M.Wt | 377.01 |
Cas No. | 856849-35-9 |
Formula | C20H21B2NO5 |
Solubility | insoluble in H2O; ≥16.65 mg/mL in DMSO; ≥4.11 mg/mL in EtOH with ultrasonic |
Chemical Name | [4-[(Z)-[(5Z)-5-[(4-boronophenyl)methylidene]-1-methyl-4-oxopiperidin-3-ylidene]methyl]phenyl]boronic acid |
SDF | Download SDF |
Canonical SMILES | B(C1=CC=C(C=C1)C=C2CN(CC(=CC3=CC=C(C=C3)B(O)O)C2=O)C)(O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Targets | Proteasome | |||||
IC50 | 1 μM |
质量控制和MSDS
- 批次:
化学结构
![AM 114](http://www.apexbt.com//media/diy/images/struct/A8163.png)
相关生物数据
![AM 114 AM 114](http://www.apexbt.com/media/diy/images/wb/A8163_1.jpg)
相关生物数据
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相关生物数据
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