3,3',5'-Triiodo-L-thyronine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
3,3',5'-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3'-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3',5'-Triiodo-L-thyronine is thyroid hormone receptors TRα and TRβ antagonist [1].
Thyroid hormones play important roles in the development of the mammalian brain by acting on migration and differentiation of neural cells, synaptogenesis, and myelination. The actions of thyroid hormones are mediated by nuclear thyroid hormone receptors (TRs) and regulation of gene expression. TRs have been also involved in adult brain function. In adult mice, TRα1 deletion and a dominant negative mutant receptor expression induce consistent behavioral changes leading to severe anxiety and morphological changes in the hippocampus [2].
The rT3 was about 1,000-fold less active at the thyroid hormone receptors TRα and TRβ compared with 3,3',5-triiodo-L-thyronine and commonly described as inactive [1].It has been reported that rT3 activates a native form of TRα, TRΔα1, which lacks a nuclear localization signal and functions in the cytoplasm. Through this action, rT3 initiates actin polymerization, particularly in astrocytes and neurons [3].
References:
[1] Nicod P, Burger A, Staeheli V, et al. A radioimmunoassay for 3, 3′, 5′-triiodo-L-thyronine in unextracted serum: method and clinical results[J]. The Journal of Clinical Endocrinology & Metabolism, 1976, 42(5): 823-829.
[2] Bernal J. Thyroid hormone receptors in brain development and function[J]. Nature clinical practice Endocrinology & metabolism, 2007, 3(3): 249-259.
[3] Senese R, Cioffi F, De Lange P, et al. Thyroid: biological actions of ‘nonclassical’thyroid hormones[J]. Journal of Endocrinology, 2014, 221(2): R1-R12.
Storage | Store at -20°C |
M.Wt | 651 |
Cas No. | 5817-39-0 |
Formula | C15H12I3NO4 |
Synonyms | Reverse T3,rT3,3,3’,5’-T3 |
Solubility | ≥32.55 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
Chemical Name | O-(4-hydroxy-3,5-diiodophenyl)-3-iodo-L-tyrosine |
SDF | Download SDF |
Canonical SMILES | IC1=C(O)C(I)=CC(OC2=CC=C(C[C@H](N)C(O)=O)C=C2I)=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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