HotStart™ 2X SYBR Green qPCR Master Mix
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
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定量PCR(qPCR,又称Real-time PCR)是一种非常通用的的精确分析基因表达的技术。按方法不同可分为染料法和探针法两类,其中染料法更通用、更方便、成本更低。基于染料的qPCR法通过实时监测结合双链DNA的染料发射的荧光,可以在PCR的每个周期间接测量DNA扩增。当在某一个时间点上,检测到的荧光信号显著超过背景,就可以确定Ct值(Cq值)。所获得的Ct值可用于评估目标基因的相对丰度,也可根据适当的标准曲线计算绝对数量。
我们的产品HotStart™ 2X SYBR Green qPCR Master Mix在定量目标DNA或cDNA方面具有优越的特异性、强劲的扩增效率、理想的可重复性和稳定性。这是一个2X PreMix,使用了结合抗体的热启动Taq DNA聚合酶。理想的Taq聚合酶和合适的缓冲液保证了较好的特异性和较高的扩增速度。Mix中的SYBR Green I可嵌入双链DNA的双螺旋小沟区域,当它与每个周期扩增的双链DNA结合时,会发出绿色荧光,通过仪器监测荧光可以实时的间接定量扩增产物。
该试剂与两种不同浓度的ROX染料一起提供,用于对应的仪器,用来校正仪器中不同反应之间的荧光信号强度。在进行实验时,使用SYBR®或SYBR/FAM模式。
Components | 1000 rxn with 10μL reaction 500 rxn with 20μL reaction 200 rxn with 50μL reaction |
5000 rxn with 10μL reaction 2500 rxn with 20μL reaction 1000 rxn with 50μL reaction |
10000 rxn with 10μL reaction 5000 rxn with 20μL reaction 2000 rxn with 50μL reaction |
HotStart™ 2X SYBR Green qPCR Master Mix | |||
50X ROX Reference Dye (low concentration) | |||
50X ROX Reference Dye (high concentration) | |||
将所有组分存放在-20°C并避光保存一年以上。尽可能避免反复冻融。 |
通过热启动机制优化扩增特异性
卓越的灵敏度和准确性
在较宽的动态范围内,Ct值具有较高的可重复性
方便的2X PreMix,简化实验步骤