Kif15-IN-1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Kif15-IN-1是一种有效的Kif15驱动蛋白抑制剂,抑制肿瘤细胞系中的细胞增殖[1]。
Kif15也称为Kinesin-12和HKLP2,是所有细胞在有丝分裂期间和后轴突神经元进行轴突生长时表达的运动蛋白[2]。Kif15是驱动蛋白相关蛋白,其有丝分裂同源物在交联和固定纺锤体微管中具有重要作用。在分裂细胞中,KIF15参与维持双极微管纺锤体,与KIF11共享冗余功能[3]。
参考文献:
[1]. Preparation of quinazolinediones as Kif15 kinesin inhibitors for treating cellular proliferative disorders. Pat. Appl. Publ. (2004), US 20040053948 A1 20040318.
[2]. Buster D W, Baird D H, Yu W, et al. Expression of the mitotic kinesin Kif15 in postmitotic neurons: implications for neuronal migration and development[J]. Journal of neurocytology, 2003, 32(1): 79-96.
[3]. Vanneste D, Takagi M, Imamoto N, et al. The role of Hklp2 in the stabilization and maintenance of spindle bipolarity[J]. Current Biology, 2009, 19(20): 1712-1717.
- 1. Geng-Yuan Chen, Fioranna Renda, et al. "Tension promotes kinetochore–microtubule release by Aurora B kinase." J Cell Biol. 2021 Jun 7;220(6):e202007030. PMID:33904910
- 2. Lee J, Cho YJ, et al. "KSP siRNA/paclitaxel-loaded PEGylated cationic liposomes for overcoming resistance to KSP inhibitors: Synergistic antitumor effects in drug-resistant ovarian cancer." J Control Release. 2020 Feb 6;321:184-197. PMID:32035195
- 3. Milic B, Chakraborty A, et al. "KIF15 nanomechanics and kinesin inhibitors, with implications for cancer chemotherapeutics." Proc Natl Acad Sci U S A. 2018 May 15;115(20):E4613-E4622. PMID:29703754
Storage | Store at -20°C |
M.Wt | 430.48 |
Cas No. | 672926-32-8 |
Formula | C20H22N4O5S |
Solubility | insoluble in H2O; insoluble in DMSO; ≥19.2 mg/mL in EtOH |
Chemical Name | (S,Z)-N-(5-(ethoxycarbonyl)-4-methylthiazol-2-yl)-2-(2-hydroxy-4-oxoquinazolin-3(4H)-yl)-3-methylbutanimidic acid |
SDF | Download SDF |
Canonical SMILES | CCOC(C(S1)=C(N=C1/N=C(O)/[C@](N2C(C3=CC=CC=C3N=C2O)=O)([H])C(C)C)C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |