o-Phenanthroline
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
O-Phenanthroline is a membrane-permeable metal chelator, known as a non-specific matrix metalloproteinase (MMP) inhibitor [1].
Matrix metalloproteinases (MMPs) are zinc-dependent proteases involved in intra- and extra-cellular matrix remodeling resulting from an oxidative stress injury to the heart, of which MMP-2 increases during myocardial ischemia-reperfusion (I/R) injury-reduced oxidative stress, proteolyzing nuclear structural proteins.
In vitro proteolysis of lamins, which are the intermediate filament proteins providing support to the nuclear structural proteins and are targets of MMP-2, o-Phenanthroline abolished the proteolysis of lamin A [2].
The isolated rat hearts were subjected to ischemia-reperfusion (I/R) injury, of which treated with o-Phenanthroline had better left ventricular developed pressure and coronary flow during the reperfusion phase compared to those did not receive the drug [2].
Reference:
[1] Georgi N, Landman E B M, Klein T J, et al. O-Phenanthroline as modulator of the hypoxic and catabolic response in cartilage tissue-engineering models [J]. J Tissue Eng Regen Med. 2017, 11(3): 724-732.
[2] Baghirova S, Hughes B G, Poirier M, et al. Nuclear matrix metalloproteinase-2 in the cardiomyocyte and the ischemic-reperfused heart [J]. J Mol Cell Cardiol. 2016, 94: 153-161.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 180.21 |
Cas No. | 66-71-7 |
Formula | C12H8N2 |
Solubility | insoluble in H2O; ≥360.42 mg/mL in DMSO; ≥58.9 mg/mL in EtOH |
Chemical Name | 1,10-phenanthroline |
SDF | Download SDF |
Canonical SMILES | C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
![o-Phenanthroline](https://www.apexbt.com//media/diy/images/struct/B7854.png)